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crat overexpression plx304 empty vector  (Addgene inc)


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    Addgene inc crat overexpression plx304 empty vector
    Crat Overexpression Plx304 Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+plx304/pLX304+(Plasmid+%2325890)/pm41216931-58-2-8
    Average 95 stars, based on 297 article reviews
    crat overexpression plx304 empty vector - by Bioz Stars, 2026-09
    95/100 stars

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    Plasmid Preparation:

    Article Title: Trichoplein binds PCM 1 and controls endothelial cell function by regulating autophagy
    Article Snippet: The scrambled sequence shRNA plasmid was purchased from Addgene, plasmid #1864. .. The packing plasmids used were pCMV‐dR8.2 dvpr, plasmid #8455 and pCMV‐VSVG, plasmid #8454 from Addgene. p62‐V5 (HsCD00434166), TCHP‐V5 (HsCD00444989) and empty vector (pLX304) are from DNASU plasmid collection, and pBABE‐puro mCherry‐EGFP‐LC3B (Addgene plasmid # 22418) and pBABEpuro GFP‐LC3 (Addgene plasmid # 22405) were a gift from Jayanta Debnath. ..

    Article Title: A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer
    Article Snippet: .. Empty vector pLX304 was obtained from Addgene. ..

    Article Title: Trichoplein controls endothelial cell function by regulating autophagy
    Article Snippet: The scrambled sequence shRNA plasmid was purchased from Addgene, plasmid #1864. .. The packing plasmids used were pCMV-dR8.2 dvpr, plasmid #8455 and pCMV-VSVG, plasmid #8454 from Addgene. p62-V5 (HsCD00434166), TCHP-V5 (HsCD00444989) and empty vector (pLX304) are from DNASU Plasmid collection, pBABE-puro mCherry-EGFP-LC3B was a gift from Jayanta Debnath (Addgene plasmid # 22418). ..



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    Soft agar colony formation in cells overexpressing WT <t>Nudt2(PLX304</t> − V5 − Nudt2). Stable overexpression of WT Nudt2 was generated in CHL − 1 melanoma cell and the cells were seeded at 5000 cells/250 µL in 24-well plates for 21 days. ( A ). Expression of endogenous Nudt2, overexpressed Nudt2 (V5 − Nudt2) was analyzed via Western blot. ( B ). Representative photomicrographs: colonies were stained with p-iodonitrotetrazolium violet stain. ( C ). Colony numbers were counted using the Image J software(ij153-win-java8); n = 7. The data are represented as mean ± SEM. For statistical analysis, Wilcoxon signed-rank test was used. * p < 0.01.
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    Soft agar colony formation in cells overexpressing WT Nudt2(PLX304 − V5 − Nudt2). Stable overexpression of WT Nudt2 was generated in CHL − 1 melanoma cell and the cells were seeded at 5000 cells/250 µL in 24-well plates for 21 days. ( A ). Expression of endogenous Nudt2, overexpressed Nudt2 (V5 − Nudt2) was analyzed via Western blot. ( B ). Representative photomicrographs: colonies were stained with p-iodonitrotetrazolium violet stain. ( C ). Colony numbers were counted using the Image J software(ij153-win-java8); n = 7. The data are represented as mean ± SEM. For statistical analysis, Wilcoxon signed-rank test was used. * p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma

    doi: 10.3390/ijms241310513

    Figure Lengend Snippet: Soft agar colony formation in cells overexpressing WT Nudt2(PLX304 − V5 − Nudt2). Stable overexpression of WT Nudt2 was generated in CHL − 1 melanoma cell and the cells were seeded at 5000 cells/250 µL in 24-well plates for 21 days. ( A ). Expression of endogenous Nudt2, overexpressed Nudt2 (V5 − Nudt2) was analyzed via Western blot. ( B ). Representative photomicrographs: colonies were stained with p-iodonitrotetrazolium violet stain. ( C ). Colony numbers were counted using the Image J software(ij153-win-java8); n = 7. The data are represented as mean ± SEM. For statistical analysis, Wilcoxon signed-rank test was used. * p < 0.01.

    Article Snippet: 1 , Empty PLX304 vector (lentiviral-expressing vector) , # 25890, Addgene Plasmid , Used to prepare Control cells for overexpression system.

    Techniques: Over Expression, Generated, Expressing, Western Blot, Staining, Software

    The lists of constructs and vectors used in this study.

    Journal: International Journal of Molecular Sciences

    Article Title: Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma

    doi: 10.3390/ijms241310513

    Figure Lengend Snippet: The lists of constructs and vectors used in this study.

    Article Snippet: 1 , Empty PLX304 vector (lentiviral-expressing vector) , # 25890, Addgene Plasmid , Used to prepare Control cells for overexpression system.

    Techniques: Construct, Plasmid Preparation, Over Expression, Expressing, Luciferase, In Vivo